Microbiological Assay of Antibiotics

[Admin]

Here is attached is the Appendix 9.1 from Indian Pharmacopoeia 1996 which is Microbiological Assay of Antibiotics.

especially Table 4 of this Appendix is important from GATE point of view as atleast 2-3 question comes from this every year.

[JOGINDER]

DRUG NAME REAGENT/INSTRUMENT

Ascorbic acid tablet----------------Ceric NH4OH oxidation
Thiamine Hcl ----------------Non aqueous titration
Pyridoxine Hcl ----------------Spectrophotometry
Ranitidine Hcl ----------------- HPLC
Sulphamethoxazole ----------------Dead stop end point
Piroxicam ---------------- HPLC
NaCl injection ---------------- Argentometry
Trimethoprim ---------------- Titration with N/10 Iodine
Riboflavine tab. ---------------- By measuring extinction at 444 nm
Paracetamol I.P. ---------------- Tetra butyl NH4OH
Phenytoin sodium I.P. ------------- HClO4
Dapsone ---------------- NaNO2
Fluorouracil ---------------- Tetra butyl NH4OH

[JOGINDER]

DRUG NAME------------- ANIMAL USED

COD LIVER OIL------------ Anaesthetised chicken
OXYTOCIN INJ.---------- Rat
HEPARIN INJ. -----------Sheep blood
INSULIN INJ.------------ Rabbit
INSULIN------------- Mice
VASOPRESSIN -----------Albino Rats
DIPHTHERIA ANTITOXIN -----------Guinea Pigs
HUMANANTIHAEMOPHYLIC----------- Sheep Plasma
Fraction

[JOGINDER]

DRUG NAME----------               MEDIUM/REAGENT ----------           INFERENCE

ARACHIS OIL-------                         U.V. LIGHT----------                              BLUE FLUORESCENCE
PALE CATECHU -----------                    NaOH & light petroleium ----------            GREEN FL.
ANTHRANOL -----------                       In alkali -----------                                 STRONG FL.
ALOE ------------                               alcoholic extract/U.V.-----------               DEEP BROWN COLOUR
BLACK CATECHU ----------                   alcoholic extract/U.V.----------                BLACK COLOUR
INDIAN RHUBARB -----------                 U.V. LIGHT-----------                             DEEP VOILET FL.
RHAPNOTIC RHUBARB-----------            U.V. LIGHT ------------                           BLUE FL.

ERGOMETRINE ----------                      WATER-----------                                  BLUE FL.

ALOE -----------                                BORAX ADD TO WATER-------------         GREEN FL.
(SCHEONTENTEN'S TEST)

CINCHONA--------                             U.V. LIGHT ------------                           PURPLE BLUE
GENTIAN ROOT ------------                                                                           WHITISH BLUE
IPOMOEA DEEP-------------                                                                           PURPLE VOILET

[SRIKANTA PANDA]

Here is attached is the Appendix 9.1 from Indian Pharmacopoeia 1996 which is Microbiological Assay of Antibiotics.

especially Table 4 of this Appendix is important from GATE point of view as atleast 2-3 question comes from this every year.